LOCUS       sequence-63583-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  TITLE     Yeast tagging plasmids.
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-yECitrine-Hygro
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..753
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 ggtcgacgga tcggtgacgg tgctggttta attaacatgt ctaaaggtga agaattattc
       61 actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct
      121 gtctccggtg aaggtgaagg tgatgctact tacggtaaat tgaccttaaa atttatttgt
      181 actactggta aattgccagt tccatggcca accttagtca ctactttagg ttatggtttg
      241 atgtgttttg ctagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg
      301 ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc
      361 agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt aaaaggtatt
      421 gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta taactctcac
      481 aatgtttaca tcatggctga caaacaaaag aatggtatca aagttaactt caaaattaga
      541 cacaacattg aagatggttc tgttcaatta gctgaccatt atcaacaaaa tactccaatt
      601 ggtgatggtc cagtcttgtt accagacaac cattacttat cctatcaatc tgccttatcc
      661 aaagatccaa acgaaaagag agaccacatg gtcttgttag aatttgttac tgctgctggt
      721 attacccatg gtattgatga attgtacaaa taaggcgcgc cacttctaaa taagcgaatt
      781 tcttatgatt tatgattttt attattaaat aagttata
//
