LOCUS       sequence-63584-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     Yeast tagging plasmids.
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-yECFP-Hygro
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..749
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYIYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 gacggatcgg tgacggtgct ggtttaatta acatgtctaa aggtgaagaa ttattcactg
       61 gtgttgtccc aattttggtt gaattagatg gtgatgttaa tggtcacaaa ttttctgtct
      121 ccggtgaagg tgaaggtgat gctacttacg gtaaattgac cttaaaattt atttgtacta
      181 ctggtaaatt gccagttcca tggccaacct tagtcactac tttaacttgg ggtgttcaat
      241 gtttttctag atacccagat catatgaaac aacatgactt tttcaagtct gccatgccag
      301 aaggttatgt tcaagaaaga actatttttt tcaaagatga cggtaactac aagaccagag
      361 ctgaagtcaa gtttgaaggt gataccttag ttaatagaat cgaattaaaa ggtattgatt
      421 ttaaagaaga tggtaacatt ttaggtcaca aattggaata catttataac tctcacaatg
      481 tttacatcac tgctgacaaa caaaagaatg gtatcaaagc taacttcaaa attagacaca
      541 acattgaaga tggttctgtt caattagctg accattatca acaaaatact ccaattggtg
      601 atggtccagt cttgttacca gacaaccatt acttatccac tcaatctgcc ttatccaaag
      661 atccaaacga aaagagagac cacatggtct tgttagaatt tgttactgct gctggtatta
      721 cccatggtat tgatgaattg tacaaataag gcgcgccact tctaaataag cgaatttctt
      781 atgatttatg atttttatta
//
