LOCUS       sequence-63585-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  TITLE     Yeast tagging plasmids.
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-yECerulean-CaUra3
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..744
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYIANSDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 atcggtgacg gtgctggttt aattaacatg tctaaaggtg aagaattatt cactggtgtt
       61 gtcccaattt tggttgaatt agatggtgat gttaatggtc acaaattttc tgtctccggt
      121 gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa aatttatttg tactactggt
      181 aaattgccag ttccatggcc aaccttagtc actactttaa cttggggtgt tcaatgtttt
      241 gctagatacc cagatcatat gaaacaacat gactttttca agtctgccat gccagaaggt
      301 tatgttcaag aaagaactat ttttttcaaa gatgacggta actacaagac cagagctgaa
      361 gtcaagtttg aaggtgatac cttagttaat agaatcgaat taaaaggtat tgattttaaa
      421 gaagatggta acattttagg tcacaaattg gaatacattg ctaactctga taatgtttac
      481 atcactgctg acaaacaaaa gaatggtatc aaagctaact tcaaaattag acacaacatt
      541 gaagatggtt ctgttcaatt agctgaccat tatcaacaaa atactccaat tggtgatggt
      601 ccagtcttgt taccagacaa ccattactta tccactcaat ctgccttatc caaagatcca
      661 aacgaaaaga gagaccacat ggtcttgtta gaatttgtta ctgctgctgg tattacccat
      721 ggtattgatg aattgtacaa ataaggcgcg ccacttctaa ataagcgaat ttcttatgat
      781 ttatgatttt tattattaaa taagttataa aaaa
//
