LOCUS       sequence-63586-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  TITLE     Yeast tagging plasmids.
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-yEPAGFP-CaUra3
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..743
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 ggatcggtga cggtgctggt ttaattaaca tgtctaaagg tgaagaatta ttcactggtg
       61 ttgtcccaat tttggttgaa ttagatggtg atgttaatgg tcacaaattt tctgtctccg
      121 gtgaaggtga aggtgatgct acttacggta aattgacctt aaaatttatt tgtactactg
      181 gtaaattgcc agttccatgg ccaaccttag tcactacttt ttcttatggt gttcaatgtt
      241 tttctagata cccagatcat atgaaacaac atgacttttt caagtctgcc atgccagaag
      301 gttatgttca agaaagaact atttttttca aagatgacgg taactacaag accagagctg
      361 aagtcaagtt tgaaggtgat accttagtta atagaatcga attaaaaggt attgatttta
      421 aagaagatgg taacatttta ggtcacaaat tggaatacaa ctataactct cacaatgttt
      481 acatcatggc tgacaaacaa aagaatggta tcaaagctaa cttcaaaatt agacacaaca
      541 ttgaagatgg ttctgttcaa ttagctgacc attatcaaca aaatactcca attggtgatg
      601 gtccagtctt gttaccagac aaccattact tatcccatca atctgcctta tccaaagatc
      661 caaacgaaaa gagagaccac atggtcttgt tagaatttgt tactgctgct ggtattaccc
      721 atggtattga tgaattgtac aaa
//
