LOCUS       sequence-63603-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Dagliyan O, Shirvanyants D, Karginov AV, Ding F, Fee L, 
            Chandrasekaran SN, Freisinger CM, Smolen GA, Huttenlocher A, Hahn 
            KM, Dokholyan NV
  TITLE     Rational design of a ligand-controlled protein conformational 
            switch.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6800-4. doi: 
            10.1073/pnas.1218319110. Epub 2013 Apr 8.
  PUBMED    23569285
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1218319110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: "6800-4"
COMMENT     Sequence Label: Tol2 Krt4 Src (YF)-uniRapR-cerulean
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..212
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     163..183
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     257..276
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        257..275
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
       61 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      121 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      181 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt
      241 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagcgtttaa
      301 acgggccctc tagactcgag cggccgccac tgtgctggat catgggcagc aacaagagca
      361 agcccaagga cgccagccag cggcgccgca gcctggagcc ctcggaaaac gtgcacgggg
      421 cagggggcgc cttcccggcc tcacagacac cgagcaagcc cgcctccgcc gacggccacc
      481 gcgggcccag cgccgccttc gtgccgcccg cggccgagcc caagctcttc ggaggcttca
      541 actcctcgga caccgtcacc tccccgcaga gggcggggcc tctggcaggt ggggtgacca
      601 cctttgtggc cctctatgac tatgagtcac ggacagagac tgacctgtcc ttcaagaaag
      661 gggagcggct gcagattgtt aacaacacgg agggagactg gtgggctggc acactcgctg
      721 agcacgggac agaccggtta catcccccag caactatgtg gcgcccctcc gactccatcc
      781 agggc
//
