LOCUS       sequence-63607-e        2988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Dagliyan O, Shirvanyants D, Karginov AV, Ding F, Fee L, 
            Chandrasekaran SN, Freisinger CM, Smolen GA, Huttenlocher A, Hahn 
            KM, Dokholyan NV
  TITLE     Rational design of a ligand-controlled protein conformational 
            switch.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6800-4. doi: 
            10.1073/pnas.1218319110. Epub 2013 Apr 8.
  PUBMED    23569285
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1218319110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: "6800-4"
COMMENT     Sequence Label: Tol2 Krt4 Src (YF)-uniRapR-cerulean
FEATURES             Location/Qualifiers
     source          1..2988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(2227..2229,2230..2232,2233..2943)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2272..2293)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2533..2552)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2880..2901
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2956..2985
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgggcagca acaagagcaa gcccaaggac gccagccagc ggcgccgcag cctggagccc
       61 tcggaaaacg tgcacggggc agggggcgcc ttcccggcct cacagacacc gagcaagccc
      121 gcctccgccg acggccaccg cgggcccagc gccgccttcg tgccgcccgc ggccgagccc
      181 aagctcttcg gaggcttcaa ctcctcggac accgtcacct ccccgcagag ggcggggcct
      241 ctggcaggtg gggtgaccac ctttgtggcc ctctatgact atgagtcacg gacagagact
      301 gacctgtcct tcaagaaagg ggagcggctg cagattgtta acaacacgga gggagactgg
      361 tggctggcac actcgctgag cacgggacag accggttaca tccccagcaa ctatgtggcg
      421 ccctccgact ccatccaggc tgaggagtgg tactttggca agatcactag acgggaatca
      481 gagcggctgc tgctcaacgc cgagaacccg agagggacct tcctcgtgag ggagagtgag
      541 accacaaaag gtgcctactg cctctctgta tccgacttcg acaatgccaa gggcctaaat
      601 gtgaaacact acaagatccg caagctggac agcggcggtt tctacatcac ctcccgcacc
      661 cagttcaaca gcctgcagca gctcgtggct tactactcca aacatgctga tggcctgtgt
      721 caccgcctca ctaccgtatg tcccacatcc aagcctcaga cccagggatt ggccaaggat
      781 gcgtgggaga tcccccggga gtccctgcgg ctggaggtca agctgggcca gggttgcttc
      841 ggagaggtgt ggatggggac ctggaacgga ccaggtacct gcgtggtgca ctacaccggg
      901 atgcttgaag atggaaagaa atttgattcc tcccgggaca gaaacaagcc ctttaagttt
      961 atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg
     1021 ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac tgggcacggt
     1081 tcgggctccg gatcaggcgt caaggacctc ctccaagcct gggacctcta ttatcatgtg
     1141 ttccgacgaa tctcaggtcc tccaggacct ggatcaggtc tctggcatga gatgtggcat
     1201 gaaggcctgg aagaggcatc tcgtttgtac tttggggaaa ggaacgtgaa aggcatgttt
     1261 gaggtgctgg agcccttgca tgctatgatg gaacggggcc cccagactct gaaggaaaca
     1321 tcctttaatc aggcctatgg tcgagattta atggaggccc aagagtggtg caggaagtac
     1381 atgaaatcag ggtcatcagg gggctccgga tcaggcatca tcccaccaca tgccactctc
     1441 gtcttcgatg tggagcttct aaaactggaa ngtcccggaa ccacaagggt tgccatcaaa
     1501 actctgaagc cangcaccat gtncccanna gncttcctgc angaggccaa ngtcatgaan
     1561 aaactgaggc acnnaaaatt ggtgcaactg tatcctgggg tntcggnann aaccatttan
     1621 attttgaaaa aattaatgaa caaggggaat cttctggnct tttncaaggg ggaaacgggc
     1681 aaatatttgc ggctacccca gctggtggac atgtctgctc agatcgcttc aggcatggcc
     1741 tatgtggagc ggatgaacta tgtgcaccgg gaccttcgag ccgccaatat cctagtaggg
     1801 gagaacctgg tgtgcaaagt ggccgacttt gggttggccc ggctcataga agacaacgaa
     1861 tacacagccc ggcaaggtgc caaattcccc atcaagtgga ccgcccctga agctgctctg
     1921 tacggcaggt tcaccatcaa gtcggatgtg tggtcctttg ggattctgct gaccgagctc
     1981 accactaagg gaagagtgcc ctatcctggg atggtgaacc gtgaggttct ggaccaggtg
     2041 gagcggggct accggatgcc ttgtcccccc gagtgccccg agtccctgca tgaccttatg
     2101 tgccagtgct ggcggaagga gcccgaggag cggcccacct tcgagtacct gcaggccttc
     2161 ctggaagact actttacgtc cactgagcca cagttccagc ccggggagaa cctaggcgga
     2221 ccaggaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2281 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga aggcgatgcc
     2341 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2401 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcgcccgcta ccccgaccac
     2461 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2521 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2581 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2641 gggcacaagc tggagtacaa cgccatcagc gacaacgtct atatcaccgc cgacaagcag
     2701 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     2761 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2821 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     2881 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     2941 aagtccggaa gcggggagca gaagctgatc tccgaggagg acctgtga
//
