LOCUS       sequence-63680-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized EGFP
            with SpHis5 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yoEGFP-SpHis5
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(69..266)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggatctgata tcatcgatga attcgagctc gtttaaactg gatggcggcg ttagtatcga
       61 atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc
      121 gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta
      181 acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg
      241 aaaacaagaa tctttttatt gtcagtactc tttacaacac tcccttcgtg cttgggactt
      301 cagaacttcc agtaagacta gtagccgcgc gcatggcaac agccagagat ttaaaagcgc
      361 tttcagcacg atgatggtca ttactaccat ataagcaggt aacatgcaaa gtaattccag
      421 ctgctaccga aaaggaatat agtaagtgag ggatcatttc acaggacaat tccccaacct
      481 tttcacgctt taatcccaaa tcgataacag catagggccg tcccgacaag tcaactacgc
      541 ttctagaaag agcttcgtca agtggacaat aagcatgtcc aaatctttta acgccggcaa
      601 agttactcat agcctgcttg aatgcaatac caagtgcaat agcagtatct tctgcagtgt
      661 gatgatcatc gatgattaaa tcacctcttg agtaaagtcg taagctccag cctgcatgtt
      721 tagccagtgc atgatacatg tgatccaaga atccaattcc cgtgtctact tggattactt
      781 gttctccctt ttggtttgta tgcttggaag ttataagttc atcaataaaa ttcgactctt
      841 caggtaaggg
//
