LOCUS       sequence-63684-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Chong T, Stary JM, Wang Y, Newmark PA
  TITLE     Molecular markers to characterize the hermaphroditic reproductive 
            system of the planarian Schmidtea mediterranea.
  JOURNAL   BMC Dev Biol. 2011 Nov 10;11:69.
  PUBMED    22074376
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2011-11-10"; volume: "11"; pages: "69"
COMMENT     Sequence Label: PL05015A1B12
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..55
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     40..56
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(642..662)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(642..660)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(681..697)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(681..697)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(694..716)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    705..721
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(729..735,736..753,754..759))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    774..795
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(912..929)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
       61 gcttgatatc gaattccatt gtgttgggca aataatggca aatatgaaag cttatcgagt
      121 cgtgaaaaag cattccaagt cacacagtca caagagactc gcaacaatcg gcatcaaaag
      181 atttgcaaat ccttattcgt tgttcttaaa gtccatgcgt actcattaca atggcgactt
      241 tgtacagttt tcccgtcagt gcgccgaaac ttggaaagta atgaattgga gtaaacggaa
      301 aaagtatgtc atgaaatctc tgaagctgaa aaagatctat gagaaggcga aaggatttac
      361 aattgttagt ccatatttaa aattcgtgtc ggagaaatat tgggagatta agaaaagcca
      421 tcccgactgg aattcaaagc aaatcaaaga cgaaacaatg caacaatata gaaatattaa
      481 aaattctaac aaaccactcg ttaattgaat ataaataatt tcttcaattg aaattaagtt
      541 tatggtaaag ataagtggat taaataaaat atttgtatgg ccaaaaaaaa aaaaaaaaaa
      601 tgcgagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
      661 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      721 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      781 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      841 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      901 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcg
//
