LOCUS       sequence-63686-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Chong T, Stary JM, Wang Y, Newmark PA
  TITLE     Molecular markers to characterize the hermaphroditic reproductive 
            system of the planarian Schmidtea mediterranea.
  JOURNAL   BMC Dev Biol. 2011 Nov 10;11:69.
  PUBMED    22074376
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2011-11-10"; volume: "11"; pages: "69"
COMMENT     Sequence Label: PL05009A1H08
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..55
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     40..56
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(754..774)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(754..772)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(793..809)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(793..809)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(806..828)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    817..833
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(841..847,848..865,866..870))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
       61 gcttgatatc gaattccatt gtgttgggca ataaactaaa agataaatgg atcgccaacg
      121 atcatggtaa atattatgaa atttcagaag agcgaaactt ttttgacgaa tgcaaaagga
      181 gcactaatgt tgtttgtcat ttttatcgag aatgtacatt tcgatgtaaa atcgttgaca
      241 aacatctcca tctcttggct aataaacatc tcgaatgtaa atttattaaa ttagacgctg
      301 aaaaatctcc attcttaaca actcggctaa atatacgagt cattcccaca atattaataa
      361 taaaaaatga aaaagccgtt gattatattg taggatttga tgatcttggt gcagtcgatg
      421 agttttctac tgaaatgatg gagtggcgaa ttgcacaggc tgatgttatt gaatattctg
      481 gtgatttgct aactcctcca gatatgaaga aagagactgt ttcttcatca atcaatcatt
      541 taagaaatcg tcgaaataag aaaactctta gagataaaac tcaagattca tccgaagatg
      601 aaaattagat ctaataaaac ctactatgaa tatattattt atattttctc ataatgtaat
      661 aatttaattt ttaaaatata gaaatgctat cagtaaaaaa aaaaaaaaaa aatgcgagcg
      721 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
      781 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      841 caacatacga gccggaagca taaagtgtaa
//
