LOCUS       sequence-63694-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     In3.4:EGFP plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJMD-In3.4:EGFP
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(303..319)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(303..319)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(356..374)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    374..398
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(497..521)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      520..529
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(571..592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(832..851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgtggcattt gtttataaca acacttcagt ggatttgaac tataccctgg agttcaaaac
       61 aaactccatg ctggcttctg ggttcttggg tgacatgttc aaggtccagt gtttgagata
      121 aggatttagt acaccattaa acaataagat aaacacttat cctggacgtg tgagcgtttt
      181 aaatactttg gcaactttaa catctttgtt tgggtacagc cttgggcaaa ggtcatttgc
      241 agatgcttga acatgtgttt gtgtctttca gaagcttgat atcgaattcc tgcagcccgg
      301 gggatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
      361 agtgagggtt aattcaagtt tgtacaaaaa agcaggctcc agtgacgtag gaagtccatc
      421 cattcacagc gcttctataa aggcgccagc tgaggcgcct actactccaa ccgcgactgc
      481 agcgagcaac tgagaaaccc agctttcttg tacaaagtgg ccaccatggt gagcaagggc
      541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      601 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      661 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      721 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca cctgtg
//
