LOCUS       sequence-63698-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized EGFP
            with Kan selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yoEGFP-Kan
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(33..35,36..746)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
ORIGIN
        1 gacggatcgg tgacggtgct ggtttaatta acatgtcaaa aggcgaggaa ctattcacag
       61 gtgtcgtacc aatactggta gaattggatg gcgacgtaaa cgggcataag ttttctgtga
      121 gtggtgaggg tgaaggggac gcaacttatg gcaaacttac acttaagttc atctgcacca
      181 cagggaagct gcctgtccca tggccaactc tagttacaac tctcacttac ggagtacaat
      241 gtttttctcg atacccagac cacatgaaac aacatgactt tttcaaatct gccatgcctg
      301 agggatatgt gcaagagaga actattttct ttaaagatga tggtaattac aaaactcgtg
      361 ctgaagtgaa gtttgaagga gatacacttg ttaatagaat tgagttaaag ggtattgact
      421 ttaaggaaga tggtaatatc ttaggtcaca aattggaata caactacaat tcacataacg
      481 tttacatcat ggcagataaa cagaaaaacg gcataaaagt taatttcaaa attagacata
      541 atatcgaaga tggttccgtt caactcgctg atcattatca acaaaataca ccaatcggag
      601 atggacctgt tctattgcca gacaaccatt acttatctac acagagcgcc ttgtcaaaag
      661 atcctaatga aaagagagat cacatggttt tgttagaatt tgtcaccgct gcgggtataa
      721 cgttgggcat ggatgaatta tacaagtaag gcgcgccact tctaaataag cgaatttctt
      781 atgatttatg atttttatta ttaaataagt tataaaaaaa
//
