LOCUS       sequence-63726-e        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, 
            Voytas DF
  TITLE     TALENs enable efficient plant genome engineering.
  JOURNAL   Plant Physiol. 2012 Nov 2.
  PUBMED    23124327
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2012 Nov 2."
COMMENT     Sequence Label: pZHY051
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(128..252)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      316..568
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(585..601)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(585..601)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(598..620)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    609..625
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(633..639,640..657,658..663))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    678..699
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(816..833)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
       61 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
      121 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
      181 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
      241 gtacaaagtg gttcgataat tcttaattaa ctagttctag agcggccgcc accgcggtgg
      301 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
      361 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
      421 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
      481 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
      541 cgcgcggtgt catctatgtt actagatcgg gaattcgtaa tcatgtcata gctgtttcct
      601 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      661 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      721 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      781 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      841 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      901 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      961 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1021 aa
//
