LOCUS       sequence-63733-e        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Dantuma NP, Groothuis TA, Salomons FA, Neefjes J
  TITLE     A dynamic ubiquitin equilibrium couples proteasomal activity to 
            chromatin remodeling.
  JOURNAL   J Cell Biol. 2006 Apr 10. 173(1):19-26.
  PUBMED    16606690
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Apr 10. 173(1):19-26."
COMMENT     Sequence Label: GFP-Ub KO.G76V
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(174..398)
                     /codon_start=1
                     /product="human ubiquitin variant in which all of the 
                     lysines have been mutated to arginine"
                     /label=ubiquitin-K0
                     /note="also known as ubiquitin-KO"
                     /translation="MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQRESTLHLVLRLRG"
     primer_bind     complement(477..498)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     826..845
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatca gttatctaga tccggtggat cccgggcccg cggtacctca cacacctctt
      181 agtcttaaga caagatgtaa ggtcgactct ctctgaatgt tgtaatcaga cagcgttcta
      241 ccgtcctcga gctgtctacc ggcaaagatc aatctttgtt gatcaggtgg aatgccttct
      301 ctgtcttgaa ttctagctct aacgttttcg atggtatcgg atggttcaac ttctagagtt
      361 atggttctac cggttaacgt tctgacgaag atctgcatat gtatataagc ttgagctcga
      421 gatctgagtc cggacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      481 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
      541 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      601 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
      661 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
      721 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      781 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      841 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
      901 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg
      961 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tg
//
