LOCUS       sequence-63737-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized mKO2
            with SpHis5 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yomKO2-SpHis5
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..686
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     primer_bind     complement(762..786)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 ggatcggtga cggtgctggt ttaattaaca tggtgtccgt aattaagcca gaaatgaaga
       61 tgaggtatta catggatggt tctgtcaatg gacacgagtt tactattgaa ggtgagggta
      121 cgggcagacc ttacgaaggc catcaagaga tgacattaag agtaactatg gctgaaggag
      181 gtccaatgcc tttcgctttc gacttagttt ctcatgtgtt ttgttacggg catcgtgtgt
      241 ttactaagta ccctgaagag ataccagatt actttaaaca agccttccca gagggacttt
      301 catgggaaag atcccttgaa tttgaagatg gagggagtgc ttcagtttct gcacatatca
      361 gcttgagagg taacacattt taccacaaat caaaattcac aggcgtcaac ttccctgccg
      421 atgggccaat catgcaaaat caatctgtcg actgggaacc atcaacagag aaaattacag
      481 catctgatgg tgttctcaaa ggtgacgtta ccatgtattt gaaactagaa ggaggtggta
      541 atcacaaatg ccaaatgaaa acaacttata aggccgctaa ggaaatattg gaaatgccag
      601 gcgaccatta catcggtcat agactggtta gaaagaccga aggcaatatc actgaacagg
      661 ttgaagatgc agtagcgcac agttaaggcg cgccacttct aaataagcga atttcttatg
      721 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
      781 tgactcttag gttttaaaac gaaaattc
//
