LOCUS       sequence-63747-e        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Jallepalli PV, Waizenegger IC, Bunz F, Langer S, Speicher MR, Peters
            JM, Kinzler KW, Vogelstein B, Lengauer C
  TITLE     Securin is required for chromosomal stability in human cells.
  JOURNAL   Cell. 2001 May 18. 105(4):445-57.
  PUBMED    11371342
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2001 
            May 18. 105(4):445-57."
COMMENT     Sequence Label: pShuttle-Cre-HA
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             196..222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YAYDVPDYA"
     polyA_signal    260..394
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(284..303)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     338..357
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(537..559)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga
       61 gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac
      121 tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat
      181 tacgatgttc cagattacgc ttacgatgtt ccagattacg ctagctaagg atagcggccg
      241 ctcgaggccg gcaaggccgg atccagacat gataagatac attgatgagt ttggacaaac
      301 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      361 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      421 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
      481 tggtatggct gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
      541 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      601 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg
      661 actcgaagat ctgggcgtgg ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt
      721 agttttgtat ctgttttgca gcagccgccg ccgccatgag caccaactcg tttgatggaa
      781 gcattgtgag ctcatatttg acaacgcgca tgcccccatg ggccggggtg cgtcagaatg
      841 tgatgggctc cagcattgat ggtcgccccg tcctgcccgc aaactctact accttgacct
      901 acgagaccgt gtctggaacg ccgttggaga ctgcagcctc cgccgccgct tcagccgctg
      961 cagccaccgc ccgcgggatt gtgactgact ttgctttcct gagcccgctt gcaagcagtg
     1021 cagct
//
