LOCUS       sequence-63758-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Burrows AE, Smogorzewska A, Elledge SJ
  TITLE     Polybromo-associated BRG1-associated factor components BRD7 and 
            BAF180 are critical regulators of p53 required for induction of 
            replicative senescence.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14280-5. doi: 
            10.1073/pnas.1009559107. Epub 2010 Jul 26.
  PUBMED    20660729
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1009559107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-08-10- 10"; volume: "107"; issue: "32"; pages: "14280-5"
COMMENT     Sequence Label: MSCV-PM-BAF180-SH1
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           63..184
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           212..226
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           254..380
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(474..493)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(475..493)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(547..570)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaatta gatctctcga ctagggataa cagggtaatt
       61 gtttgaatga ggcttcagta ctttacagaa tcgttgcctg cacatcttgg aaacacttgc
      121 tgggattact tcttcaggtt aacccaacag aaggctcgag aaggtatatt gctgttgaca
      181 gtgagcgacc atctacaagc gagttctaat agtgaagcca cagatgtatt agaactcgct
      241 tgtagatggc tgcctactgc ctcggaattc aaggggctac tttaggagca attatcttgt
      301 ttactaaaac tgaatacctt gctatctctt tgatacattt ttacaaagct gaattaaaat
      361 ggtataaatt aaatcacttt tttcaattgg aagactaatg cggccggcca gcataacttc
      421 tcgcgtgatg ttgatcaggt ggtattgtag ttatggcggt gggctgtctc gggccctata
      481 gtgagtcgta ttacctagga cgcgttctac cgggtagggg aggcgctttt cccaaggcag
      541 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
      601 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
      661 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
      721 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
      781 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
      841 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
      901 ctcaggg
//
