LOCUS       sequence-63812-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ909
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     507..529
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(567..585)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        653..757
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaagacgatg agttctactg gcgtggaatc cacaaattgc ccgagagatg ggaaaaatgt
       61 gtagctagcg acggcaaata ctttgaataa atgatttttt ctttttccac aaaatttaac
      121 gtgttttttg atttaaaaaa aacgacattt catacttgta cacctgatat ctagatacct
      181 aggtgagctc tggtaccccc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      241 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      301 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
      361 ttcacaccgc atatggtgca ctctcagtgc aatctgctct gatgccgcat agttaagcca
      421 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
      481 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
      541 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
      601 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      661 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      721 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      781 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      841 ggtgaaa
//
