LOCUS       sequence-63813-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Krupka N, Strappe P, Gotz J, Ittner LM
  TITLE     Gateway-compatible lentiviral transfer vectors for ubiquitin 
            promoter driven expression of fluorescent fusion proteins.
  JOURNAL   Plasmid. 2010 May;63(3):155-60. doi: 10.1016/j.plasmid.2010.01.002. 
            Epub 2010 Feb 2.
  PUBMED    20132838
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2010.01.002"; journalName: "Plasmid"; date: 
            "2010-05"; volume: "63"; issue: "3"; pages: "155-60"
COMMENT     Sequence Label: pLVU/RED
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    179..303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(328..333,334..351,352..358)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(478..497)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
       61 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
      121 aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatcaac
      181 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
      241 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
      301 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
      361 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
      421 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
      481 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
      541 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
      601 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
      661 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
      721 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
      781 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
      841 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
      901 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
      961 ctgatgcc
//
