LOCUS       sequence-63817-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1208
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     520..542
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(580..598)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        666..770
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atggttcgcc gcaaaagacg atgagttcta ctggcgtgga atccacaaat tgcccgagag
       61 atgggaaaaa tgtgtagcta gcgacggcaa atactttgaa taaatgattt tttctttttc
      121 cacaaaattt aacgtgtttt ttgatttaaa aaaaacgaca tttcatactt gtacacctga
      181 tatctagata cctaggtgag ctctggtacc cccctggcgt tacccaactt aatcgccttg
      241 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      301 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
      361 atctgtgcgg tatttcacac cgcatatggt gcactctcag tgcaatctgc tctgatgccg
      421 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      481 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
      541 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
      601 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      661 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      721 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      781 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      841 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      901 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
//
