LOCUS       sequence-63822-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1201
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    5..112
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     29..45
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     29..45
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(79..95)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(80..96)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(112..132)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(140..157)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(140..156)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(162..173)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agttgagctc caccgcggtg gcggccgctc tagaactagt ggatcccccg ggctgcagga
       61 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta ccaactttat
      121 tatacatagt tgataattca ctggccgtcg ttttacatgg cacgcccttc tattacaggg
      181 tttctgataa caaaccggtt attaactccc aacaagggat gatttcaatt catcatgctc
      241 aaattgaccc aaattaagtt acatgacaaa ttcatcgccc ttttcaactc ttcttggctc
      301 atcatctgtt attctgttcc tattctgtct gcaccctata cccctttgca tactctctcg
      361 tcattccttt cttggatagt gtcttcttct ccccagctct gctacttcta tgacttgcgc
      421 ccgctgcttt tccgctcgtt cctctcctct ctgacgtctt cttctgcttc ttttctcctt
      481 cctttttccc ttctcaatct tcctcttttt cccatttctg tcaatcattc gaagaagaag
      541 aagaagaaga ccctcattca tttcattttt ttttctgtcg gtgtgtgctg ctcggttaag
      601 agtgagctct ctattccacg tcttcttctt tttcttcttc gattcgaatc aatcactcca
      661 ccaaaacgca ttcgtttttg ggattcaccc cgcggttcgc aataggtttc tttttcaaat
      721 tattagcgtt ataaatagaa aaatggtgga gtttcaataa aaatgataat ttacaaagtg
      781 tattttgatt acatgtactc aaaaaggttg aaatttttca taccagtttt ccggaaatcc
      841 atctga
//
