LOCUS       sequence-63824-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1272
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    7..114
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     31..47
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     31..47
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(81..97)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(82..98)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(114..134)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(142..159)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(142..158)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(164..175)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaagttgagc tccaccgcgg tggcggccgc tctagaacta gtggatcccc cgggctgcag
       61 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccaacttt
      121 attatacata gttgataatt cactggccgt cgttttacat ggcacgccct tctattacag
      181 ggtttctgat aacaaagggt gccggatctg cggcttactc accagacctg gccccatccg
      241 attaccacct attcgcttcg atgggacacg cactcgctga gcagcgcttc gattcttacg
      301 aaagtgtgaa aaaatggctc gatgaatggt tcgccgcaaa agacgatgag ttctactggc
      361 gtggaatcca caaattgccc gagagatggg aaaaatgtgt agctagcgac ggcaaatact
      421 ttgaataaat gattttttct ttttccacaa aatttaacgt gttttttgat ttaaaaaaaa
      481 cgacatttca tacttgtaca cctgatatct agatacctag gtgagctctg gtacccccct
      541 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      601 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      661 ctgatgcggt attttctcct tacgcatctg tgcgg
//
