LOCUS       sequence-63825-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ910
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     518..540
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(578..596)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        664..768
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggttcgccgc aaaagacgat gagttctact ggcgtggaat ccacaaattg cccgagagat
       61 gggaaaaatg tgtagctagc gacggcaaat actttgaata aatgattttt tctttttcca
      121 caaaatttaa cgtgtttttt gatttaaaaa aaacgacatt tcatacttgt acacctgata
      181 tctagatacc taggtgagct ctggtacccc cctggcgtta cccaacttaa tcgccttgca
      241 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      301 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
      361 ctgtgcggta tttcacaccg catatggtgc actctcagtg caatctgctc tgatgccgca
      421 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      481 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
      541 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
      601 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
      661 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      721 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      781 catttccgtg tcgcccttat tccctttttt gcggca
//
