LOCUS       sequence-63841-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Cheeseman IM, Desai A
  TITLE     A combined approach for the localization and tandem affinity 
            purification of protein complexes from metazoans.
  JOURNAL   Sci STKE. 2005 Jan 11;2005(266):pl1.
  PUBMED    15644491
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci STKE.";
            date: "2005-01-11"; volume: "2005(266)"; pages: "pl1"
COMMENT     Sequence Label: pIC194
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             287..307
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             320..364
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     misc_feature    383..435
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    559..680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(596..615)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     650..669
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(824..845)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
       61 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      121 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
      241 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
      301 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      361 cagcggaggt ggaggtacta gtctcgagct caagcttcga attctgcagt cgacggtacc
      421 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
      481 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
      541 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      601 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      661 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
      721 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
      781 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
      841 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagg
//
