LOCUS       sequence-63848-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized mKO2
            with Kan selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yomKO2-Kan
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..681
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     primer_bind     complement(757..781)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 ggtgacggtg ctggtttaat taacatggtg tccgtaatta agccagaaat gaagatgagg
       61 tattacatgg atggttctgt caatggacac gagtttacta ttgaaggtga gggtacgggc
      121 agaccttacg aaggccatca agagatgaca ttaagagtaa ctatggctga aggaggtcca
      181 atgcctttcg ctttcgactt agtttctcat gtgttttgtt acgggcatcg tgtgtttact
      241 aagtaccctg aagagatacc agattacttt aaacaagcct tcccagaggg actttcatgg
      301 gaaagatccc ttgaatttga agatggaggg agtgcttcag tttctgcaca tatcagcttg
      361 agaggtaaca cattttacca caaatcaaaa ttcacaggcg tcaacttccc tgccgatggg
      421 ccaatcatgc aaaatcaatc tgtcgactgg gaaccatcaa cagagaaaat tacagcatct
      481 gatggtgttc tcaaaggtga cgttaccatg tatttgaaac tagaaggagg tggtaatcac
      541 aaatgccaaa tgaaaacaac ttataaggcc gctaaggaaa tattggaaat gccaggcgac
      601 cattacatcg gtcatagact ggttagaaag accgaaggca atatcactga acaggttgaa
      661 gatgcagtag cgcacagtta aggcgcgcca cttctaaata agcgaatttc ttatgattta
      721 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact
      781 cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc
      841 tttct
//
