LOCUS       sequence-63854-e       10579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Burack MA, Silverman MA, Banker G
  TITLE     The role of selective transport in neuronal protein sorting.
  JOURNAL   Neuron. 2000 May;26(2):465-72.
  PUBMED    10839364
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2000-05"; volume: "26(2)"; pages: "465-72"
COMMENT     Sequence Label: pBa.NgCam.GFP
FEATURES             Location/Qualifiers
     source          1..10579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        418..693
                     /label=chicken beta-actin promoter
     primer_bind     1626..1648
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(5567..5569,5570..5572,5573..6283)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5567..5569,5570..5572,5573..6283)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5612..5633)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5873..5892)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6220..6241
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6416..6550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6453..6472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6507..6526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6582..6598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6582..6598)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6595..6617)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6606..6622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6630..6636,6637..6654,6655..6660))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6675..6696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6813..6830)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6984..7572)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7064..7083)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7743..8534,8535..8603))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8366..8385
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8604..8708)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8776..8794
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8832..8854)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8954..8973
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9167..9189
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9181..9198
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9182..9198
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttagtgcatg catacccctc ggtgttattg ctgctctgtg cacgcacgct cattgtatca
       61 cttcatccca gtgcatgcac tcacactgga gcgattgctg ctcggtgcac gcacactcat
      121 tgtatcacgt cagctcagtg gctgcacgca caccggtgtt attgctgctc ggtgcgtgca
      181 tgcacatcag tgtcgctgca gctcagtgca tgcacgctca ttgcccatcg ctatccctgc
      241 ctctcctgct ggcgctcccc gggaggtgac ttcaagggga ccgcaggacc acctcggggg
      301 tggggggagg gctgcacacg cggaccccgc tccccctccc caacaaagca ctgtggaatc
      361 aaaaaggggg gaggggggat ggaggggcgc gtcacacccc cgccccacac cctcacctcg
      421 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      481 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
      541 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
      601 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      661 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt
      721 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      781 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
      841 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg
      901 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
      961 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1021 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga
     1081 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1141 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     1201 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     1261 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     1321 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     1381 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag
     1441 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg
     1501 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     1561 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag
     1621 ggcggggttc ggcttctggc gtgtgaccgg cggggtttat atcttccctt ctctgttcct
     1681 ccgcagcccc caagcttccg ccatggctct gccccatggg ctcggcctcc tcctgctcct
     1741 gttgctgggg gggcccggag ccgccatcac cattcccccg gagtatggtg cacacgattt
     1801 cctgcagccc cccgagctga cggaggaacc cccggaacaa ctcgtggtct tccccagtga
     1861 tgacatcgtc ctcaaatgcg tggccaccgg gaaccccccc gtccagtacc gatggagccg
     1921 tgaggatcag cccttcgtcc ccgaggagca cgggggggtc tcggtggtcc ccggatcggg
     1981 gactttggtc atcaacgcca cgttggccgc gcggctccag gggcgcttcc gctgcttcgc
     2041 caccaacgcg ttgggcaccg ctgtgtctcc cgaggccaac gtcatcgccg agaacactcc
     2101 gcagtggccg gagaagaagg tgaccccggt ggaggtggag gagggggacc ccgtggtgct
     2161 gccctgtgac ccccccgaga gcgctgtgcc ccctaaaatc tattggctca acagcgacat
     2221 cgttcacatc gctcaggacg agagggtatc gatggggcag gatgggaacc tctacttctc
     2281 caacgccatg gtgggcgaca gccaccccga ctacatctgc cacgctcact tcctcggccc
     2341 ccgcaccatc atccagaagg agcccctcga cctccgcgtg gcccccagta atgcggttcg
     2401 gtcccgccgc ccccgcctgc tgctgccccg cgacccccaa acgaccacca tcgccctccg
     2461 ggggggcagc gtcgtgttgg agtgcatcgc tgaggggctc cccactccat gggtccgatg
     2521 gcggcggctg aacggccccc tccccacccg ggcggcgttg gagaacttca acaaaacgct
     2581 gcggctgtgg ggggtgacgg agagcgacga cggggagtac gaatgtgtgg ctgagaacgg
     2641 gagggggacg gccaggggca cccacagcgt caccgtggag gccgccccat attgggtgcg
     2701 gcggccacag agtggggtct tcgggccggg ggagacggcg aggctggact gcgaggtggg
     2761 ggggaaaccc cgaccccaaa tccaatggag catcaatggg gtccccatcg acgcgctgcc
     2821 gggcgcggag cggcggtggc tgcggggcgg cgctttggtg cttccggagc tgcggccgaa
     2881 cgacagcgcg gtgctgcagt gcgaggcgag gaaccgccac ggccgcctat tggccaacgc
     2941 cttcctgcac gtcgtggagc tgccggtccg aatgctgacg gcggatgagc agcgctacga
     3001 agtggtggaa aaccaaacgg tgtttctgca ctgcagaacc ttcggggccc ccgcgccaaa
     3061 cgtcgagtgg ctgaccccca ctttggagcc ggctctgcag gacgatcgat ccttcgtgtt
     3121 caccaatggg agccttcggc tgagtgcggt gcgggggggg gacggggggg tctacacctg
     3181 catggcccaa aacgcccaca gcaacggcag cctcacggcg ctcctggagg tcagagcccc
     3241 cacccgaatt tcggcccccc cccgaagcgc caccgccaaa aaaggggaga cggtgacctt
     3301 tcactgcggg gcgacctttg accccgccgt gacccccggg gagctgcgat ggctgcgggg
     3361 ggggcagccg ctgcccgacg acccccggta ttcggtggcg gcggagacgc tgacggtgtc
     3421 caacgtggac tatggggacg aggggaccat tcagtgccgc gcctccaccc ctctcgactc
     3481 cgcggaggcc gaagcgcagc tcagagtcgt gggccgcccc ggccccatcc gggacctcca
     3541 agtgatggag gtggacgaac accgcgtgcg cctcagctgg accccggggg acgaccataa
     3601 cagccccata gagaagttcg tggtgaagga ggaggagggc atcttcagcg cggggcgctt
     3661 cgcggagcgg ctgacggttc cgggggggca gccatggacg ccccccctcc cgctgtcccc
     3721 atacgggcgg ttccgcttcc gggtggtggc cgttaacgcc tacgggaggg gggagcacag
     3781 cgcccccagc gcccccatcg agaccccccc cgcggctccg gagcgcaacc cggggggggt
     3841 ccatggggag ggcaatgaga ccggcaacct cgtcatcacc tgggagcccc tcccccccca
     3901 ggcctggaac gccccctggg ttcggtaccg cgtgcagtgg cggccattgg aggagcccgg
     3961 cggggggggc ccttcggggg ggttcccgtg ggccgaaagc accgtggacg ccccccccgt
     4021 ggtggtgggg gggctccccc cgttcagccc cttccagatc cgcgtccagg ccgtgaacgg
     4081 agccgggaag ggaccggaag cgacccccgg cgtggggcac agcggggagg acctgccgtt
     4141 ggtttaccct gagaatgtgg gggtggaact gctgaacagc agcaccgtgc gcgtgagatg
     4201 gactttgggg ggggggccca aagagctgcg ggggcgtctg aggggcttcc gggtgctgta
     4261 ttggcgtttg ggatgggtgg gggagcgcag tcgtcgacaa gccccccccg acccccccca
     4321 aatcccccaa agcccggctg aagacccacc cccatttccc cccgtggctc tgacagtggg
     4381 gggggacgcg cggggggcgc tgctgggggg gctgcggccc tggagccgtt atcagctgcg
     4441 ggtgttggtc ttcaacggga ggggggacgg cccccccagc gaacccatcg ccttcgagac
     4501 ccccgaggga gttcccggcc cccccgagga gctccgcgtg gagcggttgg acgacaccgc
     4561 cctctccgtc cattggaaac ggccgcggca tttaaacgga gtcctcacgg gatatgtact
     4621 gagataccag caggtggagc cggcgctcgg cctcccccag gaggctccgt acccccccgg
     4681 gaccctcaat gcgaccctaa gggggctgaa tgcgcgctcc cgataccggc tggcgctgcg
     4741 cgcgagcacg cgcgtcggga gcggccccgc cctgcagacg gtggggagca cgaaaccgga
     4801 accggccctc cccgctttgg agcgcgtttt ggtgtcggag gtcggggagg atttcacggt
     4861 gctgctgtgg agtttggtgc agccccagga ggacgacgtg gagttcgaag tccaattcat
     4921 gaataaaagc acggatgagc cgtggcgcac ttcgggccgc gccaactcct ctttaaggcg
     4981 gtaccgtctg gaggggctgc ggcccggcac cgcctaccga gtccaattcg tgggccggaa
     5041 ccgctccggg gaaaacgtgg ccttctggga gagcgaagtg caaaccaacg gcaccgtggt
     5101 gcgcagcctg gtgggggggt ttgccaccaa ggggtggttc atcggcttcg tcagctccgt
     5161 ggtgctcctt ctcctcatcc tcctcatcct ctgcttcatc aaacgcagca aggggggcaa
     5221 gtattcggtg aaggacaagg aggacacgca ggtggactct gaggcgcggc ccatgaagga
     5281 tgagaccttt ggggagtaca ggtcgttgga gagcgaagcg gaggagaagg gttcggcttc
     5341 gggttccggc gccggttccg gtgtgggttc tccgggtcgg ggtccgtgcg cggcgggcag
     5401 cgaagacagc ctggcggggt acggaggcag cggggatgtg cagttcaatg aggatggatc
     5461 cttcatcggg cagtaccgcg gacccggagc cggacccggc agctccggcc ctgccagccc
     5521 ctgtgctggg ccccccctgg ataagcttgg cgcgcctagg cctaccatgg tgagcaaggg
     5581 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     5641 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     5701 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     5761 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     5821 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     5881 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     5941 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     6001 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     6061 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     6121 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     6181 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     6241 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagcggaccg tctaatctag
     6301 ataagtaatg atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa
     6361 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     6421 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6481 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6541 tgtctggatc cccgggtacc gagctcgaat tcgtaatcat ggtcatagct gtttcctgtg
     6601 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6661 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6721 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6781 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6841 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6901 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6961 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7021 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7081 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7141 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7201 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7261 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7321 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7381 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     7441 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7501 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7561 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7621 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7681 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7741 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7801 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7861 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7921 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7981 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8041 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8101 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8161 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8221 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8281 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8341 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8401 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8461 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8521 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8581 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8641 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8701 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     8761 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     8821 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     8881 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggt
     8941 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     9001 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
     9061 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     9121 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     9181 tgtaaaacga cggccagtgc caagttggga tctttgcatt ggcccacggc tctcaggatg
     9241 gggatgctcc ccttcagcac ccggttcccc ttggaaactg atggtcctgg ctctgtggca
     9301 tggcagtggc actgtgagga gcccctacca gcagcacaca gtgggtttgg cactgccacg
     9361 ctccggatgc cgcgctctga tccaacccca taatcaaggg aacccgaatt gccccatcat
     9421 tgcccccacc acccccatcc tgccgggccc tcacacccca cgctgccttg tggtgacatt
     9481 ccccagccca aacccacggc ttcatggcta ccgcggggca tttcccattg ccgccccatt
     9541 atcagctctg cacacctccc gctgtaccca tgcctcgtgg ctgcccttct ttgacgtata
     9601 atcttctaat taatacccgg ccttgtcaaa gtggagcaca aacgttaatt aattccccag
     9661 caggcaggta attaacagtg tgactccctt tttgctgcga gtggggctga tacagagaga
     9721 tgtggcacta tggagcccac ggggtcctgg cactgggtgc ccacggaggt ccccatgtgc
     9781 tgcagtgtca ccgcctccga ggtgacagta ttgtccctgc ggtgtccctg cagctcagct
     9841 ctgtccacag ggccacctcc agtttggagg ggacacaatg cagccccgat gcaacccatc
     9901 ctcgcagcat cccagggaca aagaccccac tgcaagaccg cacacagggc tgggtcccgc
     9961 tcccctaata tctacagtgc ttttgcatgg ccccttaatc aatgcagtta atcagcatgc
    10021 gctcatgcac cgctctggag ctgcaaagcc cctcgcagcg ctgctcacca acaccgcgca
    10081 ccgccccggc ccagcctgca gcacgcgctg caaacaggaa agaaacaaaa tattgcccaa
    10141 atgtaggcaa aggcattcgg ctgccttgac ctccgccggg ccgggccctg cctgactcag
    10201 ctccttactc agcgctcgct tcctccctcc ggctgccacc gccgcagcgc acaccctgac
    10261 aaagagtggc ccttaacggg ctctgaggtg cacccagcag tgcactcagc agtccaaggg
    10321 ccggcctgga ggtttgcacc gctacgtgct gacattagca ttgaacttgg ccctgggtag
    10381 tgctgcaggc cgggcggggt gggtgtagag agtgcagcgc gcgttgcacc cggtgcccct
    10441 tcccctccct tgcatcccag caggctgcac cccagcacca ggcccgtgca tgcatgctcc
    10501 tggtgttatt gcagcctggt gcatgcatgc gtcttagtgg tgcagcgctg tgcatgcatc
    10561 ctccttggtg tgtagcagc
//
