LOCUS       sequence-63858-e       12109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Yang CF, Chiang MC, Gray DC, Prabhakaran M, Alvarado M, Juntti SA, 
            Unger EK, Wells JA, Shah NM
  TITLE     Sexually dimorphic neurons in the ventromedial hypothalamus govern 
            mating in both sexes and aggression in males.
  JOURNAL   Cell. 2013 May 9;153(4):896-909. doi: 10.1016/j.cell.2013.04.017.
  PUBMED    23663785
REFERENCE   2  (bases 1 to 12109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.04"; journalName: "Cell"; date: "2013-05-9- 9";
            volume: "153"; issue: "4"; pages: "896-909"
COMMENT     Sequence Label: pLenti-lxlplap
FEATURES             Location/Qualifiers
     source          1..12109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2434..2551
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2605..3815
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3652..3671
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3853..3862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3865..3898)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3907..4287
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MAPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYK
                     VLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLL
                     PGELAKHAVSEGTKAVTKYTSAK"
     regulatory      4300..4309
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4306..4308,4309..4311,4312..5025)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4306..4308,4309..4311,4312..5022)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4351..4372)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4612..4631)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4959..4980
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5165..5198)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5213..6712
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLGERQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     misc_feature    6809..7397
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6862..6882)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7280..7291)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7910..8090
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8116..8133)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8122..8346
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8392..8820
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8479..8498)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8689..8710
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8829..8849)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8834..9164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9015..9150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9077..9096
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9212..9259
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9278..9652
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9782..9903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9819..9838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9873..9892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9952..9968)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9952..9968)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9965..9987)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9976..9992
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10000..10006,10007..10024,10025..10030))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10045..10066
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10183..10200)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10354..10942)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10434..10453)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11113..11904,11905..11973))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11736..11755
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11974..12078)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     2401 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2461 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2521 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2581 agtttggtta attaagggtg cagcggcctc cgcgccgggt tttggcgcct cccgcgggcg
     2641 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcaggagc gttcctgatc
     2701 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     2761 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     2821 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     2881 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     2941 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     3001 tggtgagttg cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     3061 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     3121 ctgaactggg ggttgggggg agcgcacaaa atggcggctg ttcccgagtc ttgaatggaa
     3181 gacgcttgta aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     3241 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     3301 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcgggttt
     3361 gtcgtctggt tgcgggggcg gcagttatgc ggtgccgttg ggcagtgcac ccgtaccttt
     3421 gggagcgcgc gcctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     3481 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     3541 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     3601 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     3661 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     3721 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3781 ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttgggctgca ggtcgactct
     3841 agtaccggtg ccgccaccat gggtataact tcgtatagca tacattatac gaagttatgg
     3901 cgcgccatgg caccagagcc agcgaagtct gctcccgccc cgaaaaaggg ctccaagaag
     3961 gcggtgacta aggcgcagaa gaaaggcggc aagaagcgca agcgcagccg caaggagagc
     4021 tattccatct atgtgtacaa ggttctgaag caggtccacc ctgacaccgg catttcgtcc
     4081 aaggccatgg gcatcatgaa ttcgtttgtg aacgacattt tcgagcgcat cgcaggtgag
     4141 gcttcccgcc tggcgcatta caacaagcgc tcgaccatca cctccaggga gatccagacg
     4201 gccgtgcgcc tgctgctgcc tggggagttg gccaagcacg ccgtgtccga gggtactaag
     4261 gccgtcacca agtacaccag cgctaaggat ccaccggtcg ccaccatggt gagcaagggc
     4321 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4381 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4441 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     4501 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     4561 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     4621 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     4681 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4741 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     4801 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     4861 aacactccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     4921 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4981 accgccgccg ggatcactct cggcatggac gagctgtaca agtaatagga attccagctg
     5041 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg
     5101 ggggatctaa gctctagttg attgactaac tagctgatag ctgactaact gcaggggccc
     5161 tcgaataact tcgtatagca tacattatac gaagttatgt cgacctcgag gactgctgct
     5221 gctgctgctg ctgggcctga ggctacagct ctccctgggc atcatcccag ttgaggagga
     5281 gaacccggac ttctggaacc gcgaggcagc cgaggccctg ggtgccgcca agaagctgca
     5341 gcctgcacag acagccgcca agaacctcat catcttcctg ggcgatggga tgggggtgtc
     5401 tacggtgaca gctgccagga tcctaaaagg gcagaagaag gacaaactgg ggcctgagat
     5461 acccctggcc atggaccgct tcccatatgt ggctctgtcc aagacataca atgtagacaa
     5521 acatgtgcca gacagtggag ccacagccac ggcctacctg tgcggggtca agggcaactt
     5581 ccagaccatt ggcttgagtg cagccgcccg ctttaaccag tgcaacacga cacgcggcaa
     5641 cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg aagtcagtgg gagtggtaac
     5701 caccacacga gtgcagcacg cctcgccagc cggcacctac gcccacacgg tgaaccgcaa
     5761 ctggtactcg gacgccgacg tgcctgcctc ggcccgccag gaggggtgcc aggacatcgc
     5821 tacgcagctc atctccaaca tggacattga tgtgatccta ggtggaggcc gaaagtacat
     5881 gtttcgcatg ggaaccccag accctgagta cccagatgac tacagccaag gtgggaccag
     5941 gctggacggg aagaatctgg tgcaggaatg gctcggcgaa cgccagggtg cccggtacgt
     6001 gtggaaccgc actgagctca tgcaggcttc cctggacccg tctgtgaccc atctcatggg
     6061 tctctttgag cctggagaca tgaaatacga gatccaccga gactccacac tggacccctc
     6121 cctgatggag atgacagagg ctgccctgcg cctgctgagc aggaaccccc gcggcttctt
     6181 cctcttcgtg gagggtggtc gcatcgacca tggtcatcat gaaagcaggg cttaccgggc
     6241 actgactgag acgatcatgt tcgacgacgc cattgagagg gcgggccagc tcaccagcga
     6301 ggaggacacg ctgagcctcg tcactgccga ccactcccac gtcttctcct tcggaggcta
     6361 ccccctgcga gggagctcca tcttcgggct ggcccctggc aaggcccggg acaggaaggc
     6421 ctacacggtc ctcctatacg gaaacggtcc aggctatgtg ctcaaggacg gcgcccggcc
     6481 ggatgttacc gagagcgaga gcgggagccc cgagtatcgg cagcagtcag cagtgcccct
     6541 ggacgaagag acccacgcag gcgaggacgt ggcggtgttc gcgcgcggcc cgcaggcgca
     6601 cctggttcac ggcgtgcagg agcagacctt catagcgcac gtcatggcct tcgccgcctg
     6661 cctggagccc tacaccgcct gcgacctggc gccccccgcc ggcaccaccg acgccgcgca
     6721 cccggggcgg tccgtggtcc ccgcgttgct tcctctgctg gccgggaccc tgctgctgct
     6781 ggagacggcc actgctccct gactcgagaa tcaacctctg gattacaaaa tttgtgaaag
     6841 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6901 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6961 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7021 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7081 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7141 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7201 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7261 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7321 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7381 ttgggccgcc tccccgcctg gtcgagacct ggaaaaacat ggagcaatca caagtagcaa
     7441 cacagcagct accaatgctg cttgtgcctg gctagaagca caagaggagg aggaggtggg
     7501 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     7561 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aaagaagaca
     7621 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt gacagaacta
     7681 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     7741 agttgagcca gataagatag aagaggccaa taaaggagag aacaccagct tgttacaccc
     7801 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     7861 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gactgtactg ggtctctctg
     7921 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7981 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8041 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     8101 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     8161 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     8221 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     8281 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     8341 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     8401 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     8461 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     8521 gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac
     8581 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     8641 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     8701 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     8761 tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     8821 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca
     8881 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     8941 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     9001 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9061 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     9121 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     9181 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     9241 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     9301 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     9361 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     9421 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     9481 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     9541 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     9601 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     9661 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     9721 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     9781 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9841 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9901 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     9961 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    10021 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    10081 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    10141 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    10201 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10261 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10321 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10381 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10441 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10501 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
    10561 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10621 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10681 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10741 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    10801 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10861 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10921 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    10981 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    11041 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    11101 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    11161 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    11221 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    11281 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    11341 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    11401 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    11461 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    11521 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    11581 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    11641 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    11701 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    11761 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11821 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11881 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    11941 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    12001 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    12061 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
