LOCUS       sequence-63870-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized 
            mRuby2 with SpHis5 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yomRuby2-SpHis5
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..740
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 ggatcggtga cggtgctggt ttaattaaca tggtgtccaa aggagaggag ttaatcaagg
       61 aaaacatgag aatgaaagtt gtcatggagg gctccgttaa tggtcaccaa ttcaagtgta
      121 caggggaagg tgaaggtaat ccttacatgg gtacacaaac tatgagaatt aaagtaattg
      181 aaggcggacc actaccattt gcatttgaca ttctggcaac gtcattcatg tacggatcac
      241 gaactttcat caagtaccct aaaggtatac cagacttttt caagcaatct tttccagagg
      301 gttttacatg ggaaagggtt acaagatacg aagatggggg tgtcgtcaca gttatgcaag
      361 atacttcatt agaagatggc tgccttgtct atcatgtgca agtaagaggg gtgaattttc
      421 cttctaacgg acctgtgatg cagaaaaaga ccaaaggttg ggaaccaaat actgaaatga
      481 tgtacccagc tgatggaggt ttgagaggct acacacacat ggcgcttaaa gttgatggtg
      541 gaggtcattt gtcttgtagt tttgttacca cttatcgttc taaaaagact gttggcaata
      601 tcaaaatgcc aggaatacat gctgtagacc acagactaga aagactcgaa gagagcgata
      661 acgaaatgtt cgttgtacag agagagcatg ccgtagccaa atttgctggc ttaggcggtg
      721 gtatggatga attgtataag taaggcgcgc cacttctaaa taagcgaatt tcttatgatt
      781 tatgattttt attattaaat aagttataaa aaa
//
