LOCUS       sequence-63878-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     Oshima lab unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FNpCDNA3
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             77..100
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             101..121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(213..231)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(214..231)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(251..268)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    257..481
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      527..885
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(614..633)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     824..845
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc accatggact acaaggacga cgacgataag ccaaagaaaa agcgaaaggt
      121 cggatccact agtaacggcc gccagtgtgc tggaattctg cagatatcca tcacactggc
      181 ggccgctcga gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct
      241 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      301 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      361 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
      421 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
      481 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
      541 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      601 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      661 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      721 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      781 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      841 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattt
//
