LOCUS       sequence-63880-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Sehnke PC, Laughner B, Cardasis H, Powell D, Ferl RJ
  TITLE     Exposed loop domains of complexed 14-3-3 proteins contribute to 
            structural diversity and functional specificity.
  JOURNAL   Plant Physiol. 2006 Feb;140(2):647-60. Epub 2006 Jan 11.
  PUBMED    16407442
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006-02"; volume: "140(2)"; pages: "647-60. Epub 
            2006 Jan 11"
COMMENT     Sequence Label: pET15b-At 14-3-3 phi
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggcggca ccaccagcat catcctcggc gagggaagag
      121 ttcgtgtacc tcgcaaagct cgcagagcaa gcggaacgtt acgaagaaat ggttgaattc
      181 atggaaaaag tcgctgaagc cgttgacaaa gacgaactca ccgtcgaaga acgtaatctc
      241 ctctccgtcg cttacaaaaa cgtaatcggc gctcgtcgtg cttcctggag aatcatctct
      301 tccattgaac aaaaagaaga gagtcgtggt aacgatgacc atgtgaccac gatccgtgat
      361 tacagaagca agatcgaatc tgagttatcg aaaatctgtg acggtattct taagcttctt
      421 gatactagac ttgttcctgc ttctgctaat ggagattcta aggtttttta ccttaagatg
      481 aagggagatt atcataggta tttggctgag tttaagactg gtcaagagag gaaagatgct
      541 gctgaacata ctctcaccgc ttacaaagct gctcaggata ttgctaatgc tgaattggct
      601 ccaacgcatc cgattcgtct tggtttggct ttgaatttct ctgtgtttta ctatgagatt
      661 cttaattctc cagatcgtgc ttgtaatctc gctaagcagg cgtttgatga agccattgct
      721 gagttagata ctcttggaga ggagtcatac aaggatagta ccttgatcat gcagcttctt
      781 cgtgacaatc ttactctctg gacttctgac atgcaggacg aaagtccgga ggagattaaa
      841 gaagcagcag caccaaagcc tgctgaa
//
