LOCUS       sequence-63884-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized 
            mKeima with Kan selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yomKeima-Kan
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(781..799)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(782..799)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tcataaatca taagaaattc gcttatttag aagtggcgcg ccttacccta atagagaatg
       61 tcttgcgata gcaatttcgc attgttcgac ggatgtgtaa tctctgttgt ggctagtaac
      121 atccaactta cggtcgatct cgtgtctgcc aggcattctg acaggctttt tcgccttata
      181 agttgactta aactcacata agtaatgacc acccccttcc agctttagtg ccatgtagtc
      241 gttaccaata agcataccat ctctggcaaa taacctttca gtagatggtt cccacccctg
      301 tgttttcttt tgcattactg gaccatttgg aggaaaattc tcgcctgaaa tcttaacgtt
      361 gtagataaaa caattgccct gtatagaaga gtcatttgac accgtacaaa cagcaccatc
      421 ttcgaaattc atggatctct cccatgtata tccttctggg aaagattgtt taaagtagtc
      481 tggaatatct tcaggatatt ttgtgaatgg tatactaccg tattgcaatt gaggtgaaag
      541 gatatcccag gcaaatggga gtggtcctcc tttggtaaca gtcaatttca cggtttgttc
      601 gccttcgtat ggttttcctt taccatcacc ttctacctcg aagtaatggc cgtttactgt
      661 tccactcatg tagactttat aagtcatttg tttagcgatc acagaaacca tgttaattaa
      721 accagcaccg tcaccgatcc gtcgacctgc agcgtacgaa gcttcagctg gcggccgcgt
      781 tctatagtgt cacctaaatc gtatgtgtat gatac
//
