LOCUS       sequence-63887-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Pijnappel WW, Esch D, Baltissen MP, Wu G, Mischerikow N, Bergsma AJ,
            van der Wal E, Han DW, Bruch Hv, Moritz S, Lijnzaad P, Altelaar AF, 
            Sameith K, Zaehres H, Heck AJ, Holstege FC, Scholer HR, Timmers HT
  TITLE     A central role for TFIID in the pluripotent transcription circuitry.
  JOURNAL   Nature. 2013 Mar 28;495(7442):516-9. doi: 10.1038/nature11970. Epub 
            2013 Mar 17.
  PUBMED    23503660
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11970"; journalName: "Nature"; date: "2013-03-28- 
            28"; volume: "495"; issue: "7442"; pages: "516-9"
COMMENT     Sequence Label: pMX-mTAF4b
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             197..262
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             281..394
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 catcctctag actgccggat ctagctagtt aattaaggat cccagtgtgg tggtacggga
       61 attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata
      121 accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttaaa
      181 ctcgagcggc cgcatggact acaaagacca tgacggtgat tataaagatc atgacatcga
      241 ctacaaggat gacgatgaca agggaagcgg tagcggtacc atggacgaga agaccaccgg
      301 ctggcggggc ggccacgtgg tggagggcct ggccggcgag ctggagcagc tgcgggccag
      361 gctggagcac caccctcagg gccagcggga gccctccggc ggctgcaagc tgggcgcccg
      421 ggcggatccc atgccggcgg gcctcacgga gccggccggc gccgctgctc ctgtagtctc
      481 gagcgcgtcg ggggccgtga ccatggctcc ggtcgccgcg ctgcccgtcc gggtggaggg
      541 caccccggtg gccctgggcc ctgtgactaa ggctcctgtc agtgtttgcg tggagtctgt
      601 ggcgccccag cccttgccag ccccagtggg gactctagtg accaaagtgg ttcctgtcac
      661 tgcccttcct aaactcggca gccctaggct gcccgctcct cagatagtca ccgtgaaaac
      721 gccgggcacc acgacaatcc aactaccggc taatttgcag cttcctccag gaacagtttt
      781 gatcaaaagt aacagtggtc agttgatgct ggtatcgcct cagcaagctg taacaggagc
      841 caagaccaca agtaacataa ctccaagacc agcagtacca gcaaacactc aaacagtaaa
      901 gatctgtaca gtgccgaatt ctagctcaca attaatgaag aaagtaacag tggcgcctgt
      961 taaaaatttg acaca
//
