LOCUS       sequence-63890-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Gascoigne KE, Takeuchi K, Suzuki A, Hori T, Fukagawa T, Cheeseman IM
  TITLE     Induced ectopic kinetochore assembly bypasses the requirement for 
            CENP-A nucleosomes.
  JOURNAL   Cell. 2011 Apr 29;145(3):410-22. doi: 10.1016/j.cell.2011.03.031.
  PUBMED    21529714
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2011.03"; journalName: "Cell"; date: "2011-04-29- 
            29"; volume: "145"; issue: "3"; pages: "410-22"
COMMENT     Sequence Label: pKG209
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             81..125
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 agtccggagg ggatcggtgg agctccaccg gtggcggccg ctctagagag aatctttatt
       61 ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg
      121 acagcggagg tggaggtact agtctcgaga tggcagacca caaccctgac agcgattcta
      181 cacctcgtac actgctgcgt cgtgtgctgg atactgctga tcctcgtact cctcgtcgtc
      241 ctcgtagcgc tcgtgctggt gctcgccgtg ccctgctgga aacagcctct cctcgtaaac
      301 tgagtggcca gacccgtact attgcccgtg ggcgttcaca tggagcccgc tcggtcggtc
      361 gtagtgctca cattcaagcc tcgggacacc tggaagaaca aacccctcgt accctgctga
      421 aaaacatcct gctgactgcc cctgaaagct ctattctgat gccggaaagc gtcgttaaac
      481 cggttcctgc tcctcaagcc gttcaacctt ctcgtcaaga gtcctcctgt ggatctctgg
      541 agctgcaact gcctgaactg gagcctccta caacactggc accggggctg ctggcgcctg
      601 gtcgtcgtaa acaacgtctg cgtctgagcg tgtttcaaca aggtgttgat cagggcctga
      661 gtctgtctca agaacctcaa ggcaacgctg atgcttctag tctgacccgt tctctgaacc
      721 tgacctttgc tactccactg caacctcaaa gcgtccaacg tcctggactg gctcgccgcc
      781 ctcctgctcg ccgtgccgtt gatgtgggtg cttttctgcg tgacctgcgt gatacaagcc
      841 tggcacctcc gaatattgtg ctggaggata cacagccgtt tt
//
