LOCUS       sequence-63892-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Gascoigne KE, Takeuchi K, Suzuki A, Hori T, Fukagawa T, Cheeseman IM
  TITLE     Induced ectopic kinetochore assembly bypasses the requirement for 
            CENP-A nucleosomes.
  JOURNAL   Cell. 2011 Apr 29;145(3):410-22. doi: 10.1016/j.cell.2011.03.031.
  PUBMED    21529714
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2011.03"; journalName: "Cell"; date: "2011-04-29- 
            29"; volume: "145"; issue: "3"; pages: "410-22"
COMMENT     Sequence Label: pKG215
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             81..125
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(172..191)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 agtccggagg ggatcggtgg agctccaccg gtggcggccg ctctagagag aatctttatt
       61 ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg
      121 acagcggagg tggaggtact agtctcgaga tggtgaaacc agtaacgtta tacgatgtcg
      181 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
      241 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
      301 atcgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
      361 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
      421 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
      481 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
      541 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
      601 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
      661 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
      721 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
      781 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
      841 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
      901 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
      961 tagtgggata c
//
