LOCUS       sequence-63896-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  TITLE     pET15b-At 14-3-3 omicron
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b-At 14-3-3 omicron
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggagaac gagagagcga agcaagtgta cttggcgaaa
      121 ctcaacgagc aagctgagag atacgatgag atggttgaag caatgaagaa agttgcagct
      181 cttgatgtag agctcaccat tgaagagagg aatcttttat ctgttggtta caaaaatgtg
      241 attggtgcta ggagagcatc gtggaggatc ctttcttcga tcgagcagaa agaagaatcg
      301 aaaggaaacg aacaaaatgc caagaggatt aaggattaca ggacaaaagt tgaagaagaa
      361 ctctccaaga tttgttacga tattttggct gtcatcgata agcatcttgt cccattcgca
      421 acttcgggag aatccaccgt cttctactac aaaatgaaag gagattattt cagatacttg
      481 gctgaattta agtccggtgc tgatcgcgag gaagctgcgg acctatcact caaggcgtat
      541 gaagctgcaa cgagttctgc tagcacggaa ttgtctacaa cacatccaat aagactcggc
      601 ttggctctca acttttctgt cttctactac gagatcttga attctccgga aagagcatgt
      661 catttggcaa agcgagcgtt tgatgaagca atcgctgagc ttgacagtct taacgaagat
      721 tcatacaagg acagcacact tatcatgcaa cttctcagag ataacctcac tttatggacc
      781 tctgatctag aggaaggagg tgaacaatct aaaggccata atcaacaaga tgagaactaa
      841 ctcgaggatc cggctgctaa caaagcccga aaggaagctg agt
//
