LOCUS       sequence-63898-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Wu K, Lu G, Sehnke P, Ferl RJ
  TITLE     The heterologous interactions among plant 14-3-3 proteins and 
            identification of regions that are important for dimerization.
  JOURNAL   Arch Biochem Biophys. 1997 Mar 1;339(1):2-8.
  PUBMED    9056226
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Biochem Biophys."; date: "1997-03-1"; volume: "339(1)"; pages: "2-8"
COMMENT     Sequence Label: pET15b-At 14-3-3 omega
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggcgtct gggcgtgaag agttcgtata catggctaag
      121 ctcgcggagc aagcggagag gtacgaagag atggtagagt ttatggagaa agtctccgcc
      181 gccgttgatg gcgatgaact caccgtagaa gagcgaaatc ttctctccgt cgcttataag
      241 aatgtgattg gtgctcgccg tgcctcgtgg cgtatcattt catcgatcga gcagaaggaa
      301 gagagccgtg gtaacgatga ccacgtcacg gcgatccgtg aatataggtc taagatcgag
      361 acggaactct ccggaatctg cgacggaatc cttaagttgc ttgactctag actcatccct
      421 gccgctgctt ctggtgattc caaggtcttt taccttaaga tgaagggaga ttatcacagg
      481 tacttggctg agtttaagac tggtcaagag aggaaagacg ccgccgaaca tacactcgcc
      541 gcttacaaat ctgctcagga tattgctaat gcagagcttg ctccaacaca cccaattcgt
      601 cttggtcttg cattgaactt ctctgtgttc tattacgaga tcctcaattc tcctgatcgt
      661 gcctgtaacc tcgccaaaca ggcctttgat gaggcgattg cagagttgga cactcttggt
      721 gaagagtcat acaaagacag taccttgatc atgcagcttc ttcgtgacaa tctcactctc
      781 tggacatctg atatgcagga tgatgctgcg gatgagatca aggaagcagc agcgccaaaa
      841 ccgaccgagg aacagcagtg aattccaagc cgaattc
//
