LOCUS       sequence-63899-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Wu K, Lu G, Sehnke P, Ferl RJ
  TITLE     The heterologous interactions among plant 14-3-3 proteins and 
            identification of regions that are important for dimerization.
  JOURNAL   Arch Biochem Biophys. 1997 Mar 1;339(1):2-8.
  PUBMED    9056226
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Biochem Biophys."; date: "1997-03-1"; volume: "339(1)"; pages: "2-8"
COMMENT     Sequence Label: pET15b-At 14-3-3 psi
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgtcgaca agggaagaga atgtttacat ggcgaaatta
      121 gccgaacaag ctgaacgtta cgaagaaatg gttgaattca tggagaaagt tgcgaaaact
      181 gttgatgttg aggaactttc agttgaagag aggaatcttc tctctgttgc ttacaagaac
      241 gtgattggag cgagaagagc ttcgtggaga atcatttctt cgattgagca gaaagaagag
      301 agcaaaggga acgaagatca tgttgctatt atcaaggatt acagaggaaa gattgaatcc
      361 gagcttagca aaatctgtga tgggattttg aatgttcttg aagctcatct tattccttct
      421 gcttcaccag ctgaatctaa agtgttttat cttaagatga agggtgatta tcataggtat
      481 cttgctgagt ttaaggctgg tgctgaaagg aaagaagctg ctgaaagcac tttggttgct
      541 tacaagtctg cttccgacat tgccactgct gagttagctc ctactcaccc gataaggctt
      601 ggtcttgcac tcaacttctc tgtgttttac tatgaaatcc tcaactcgcc tgatcgtgct
      661 tgcagcctcg caaagcaggc gtttgatgat gcaatcgctg agttagatac attgggtgag
      721 gaatcataca aggacagtac actgattatg cagcttctta gagacaatct cactctctgg
      781 acttcagata tgactgacga agcaggagat gagattaagg aggcatcaaa gcccgatggt
      841 gccgagtaaa cccgaagaa
//
