LOCUS       sequence-63900-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Wu K, Lu G, Sehnke P, Ferl RJ
  TITLE     The heterologous interactions among plant 14-3-3 proteins and 
            identification of regions that are important for dimerization.
  JOURNAL   Arch Biochem Biophys. 1997 Mar 1;339(1):2-8.
  PUBMED    9056226
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Biochem Biophys."; date: "1997-03-1"; volume: "339(1)"; pages: "2-8"
COMMENT     Sequence Label: pET15b-At 14-3-3 upsilon
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..72
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag cggcctggtg
       61 ccgcgcggca gccatatgtc ttctgattcg tcccgggaag agaatgtgta cttggccaag
      121 ttagccgagc aagctgagcg ttacgaggaa atggttgagt tcatggagaa agttgcaaag
      181 accgtggaga ccgaggaact tactgttgaa gagaggaatc tcttgtctgt tgcttacaag
      241 aacgtgattg gtgctaggag agcttcttgg aggattatct cttccattga gcagaaggaa
      301 gatagcaggg gcaacagtga tcatgtttcg attatcaagg attacagagg caagattgaa
      361 actgagctca gcaagatttg tgatggcatt ttgaaccttc ttgaggctca tctcattcct
      421 gctgcttctt tggctgagtc caaagttttt tacctgaaga tgaagggaga ttatcatcgg
      481 taccttgctg aattcaagac tggtgctgag aggaaagaag ctgctgagag cactcttgtt
      541 gcctacaagt ctgctcagga tattgctctt gctgatctgg ctcccactca cccaatcaga
      601 ctggggcttg ctcttaactt ctctgttttc tactatgaga ttctcaactc atctgatcgt
      661 gcgtgtagtc tcgcaaagca ggcttttgat gaggcaatct cggagctaga cacattggga
      721 gaggaatcat acaaggacag tacattgatc atgcagcttc tccgtgacaa tctcaccctc
      781 tggacttctg acctcaatga cgaagctggt gatgatatca aggaagcccc gaaagaggtg
      841 cagaaagttg atgaacaagc ccaaccacca ccttcgca
//
