LOCUS       sequence-63901-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Sehnke PC, Laughner B, Cardasis H, Powell D, Ferl RJ
  TITLE     Exposed loop domains of complexed 14-3-3 proteins contribute to 
            structural diversity and functional specificity.
  JOURNAL   Plant Physiol. 2006 Feb;140(2):647-60. Epub 2006 Jan 11.
  PUBMED    16407442
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006-02"; volume: "140(2)"; pages: "647-60. Epub 
            2006 Jan 11"
COMMENT     Sequence Label: pET15b-At 14-3-3 epsilon
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggagaat gagagggaaa agcaggttta cttggctaag
      121 ctctccgagc aaaccgaaag atacgatgaa atggtggagg cgatgaagaa agttgctcag
      181 cttgatgtgg agctaactgt ggaagagagg aatcttgtat ctgtagggta caagaatgtg
      241 attggtgcaa ggagagcatc atggagaata ctatcttcca ttgagcagaa ggaagagtcc
      301 aagggaaatg atgaaaatgt caagaggctt aagaattatc gtaagagagt tgaagatgag
      361 cttgctaaag tttgtaatga catcttgtct gtcattgata agcatctcat tccatcgtct
      421 aacgctgtgg agtcaactgt ctttttctac aaaatgaaag gagattacta tcgctatctt
      481 gcggagttca gttctggtgc tgaacgcaag gaagctgcag atcagtctct tgaagcatat
      541 aaggctgctg ttgctgctgc agagaatggt ttggcaccca cacatccagt tagacttggc
      601 ttggcgttga acttttcagt tttctactat gagatcttga actctcccga aagcgcatgc
      661 caattggcta agcaagcatt cgatgatgca attgctgaac ttgacagcct caacgaggaa
      721 tcatacaaag acagcactct tattatgcag ctacttagag acaatctcac cttgtggact
      781 tcagacctta atgaggaagg agatgagaga accaaaggtg ctgatgagcc tcaagatgag
      841 aactaaatcc tctgtgagaa gagaaacgac t
//
