LOCUS       sequence-63902-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Sehnke PC, Laughner B, Cardasis H, Powell D, Ferl RJ
  TITLE     Exposed loop domains of complexed 14-3-3 proteins contribute to 
            structural diversity and functional specificity.
  JOURNAL   Plant Physiol. 2006 Feb;140(2):647-60. Epub 2006 Jan 11.
  PUBMED    16407442
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006-02"; volume: "140(2)"; pages: "647-60. Epub 
            2006 Jan 11"
COMMENT     Sequence Label: pET15b-At 14-3-3 iota
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgtcatca tcaggatccg acaaagagag agagactttt
      121 gtctatatgg ctaagctctc cgaacaagcc gagcgatacg acgagatggt ggagacgatg
      181 aagaaagtgg cgagggttaa tagcgagctg actgtggaag agaggaatct gttgtcggtt
      241 ggttacaaga acgtgatcgg agcaagaaga gcttcgtgga ggataatgtc gtccatcgag
      301 cagaaagaag agtctaaagg caatgaatca aacgtgaaac aaatcaaggg ttatcgccaa
      361 aaggtagaag atgagctcgc taacatttgc caagacattc tcaccatcat tgatcagcat
      421 ctcatccctc acgctacctc aggggaagct accgtcttct actacaagat gaaaggagat
      481 tattaccgtt acttggctga gtttaagacc gagcaagaga ggaaggaagc tgctgaacag
      541 tctctcaaag gctatgaggc tgcaacacaa gctgcaagca ctgagcttcc ttcgacccat
      601 ccaattcgtc ttggccttgc tcttaacttc tctgttttct actatgagat catgaactct
      661 cctgaaagag cttgccattt ggctaagcaa gccttcgatg aggcgattgc agagttagat
      721 actctaagtg aggagtcgta caaggacagc actttgatca tgcagctcct gagagacaac
      781 ctcaccctct ggacttctga tcttcctgaa gatggaggag aagacaacat caagactgaa
      841 gaatccaagc aagaacaggc caagc
//
