LOCUS       sequence-63904-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Sehnke PC, Laughner B, Cardasis H, Powell D, Ferl RJ
  TITLE     Exposed loop domains of complexed 14-3-3 proteins contribute to 
            structural diversity and functional specificity.
  JOURNAL   Plant Physiol. 2006 Feb;140(2):647-60. Epub 2006 Jan 11.
  PUBMED    16407442
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006-02"; volume: "140(2)"; pages: "647-60. Epub 
            2006 Jan 11"
COMMENT     Sequence Label: pET15b-At 14-3-3 nu
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgtcgtct tctcgggaag agaatgtgta cttagccaag
      121 ttagctgagc aagctgaacg ttatgaggaa atggttgagt tcatggagaa agttgcaaag
      181 actgttgaca ccgatgagct tactgtcgaa gagagaaacc tcttgtctgt tgcttacaag
      241 aacgtcattg gtgctaggag agcttcctgg aggatcatat cttccattga acagaaggaa
      301 gaaagcagag gaaacgatga tcatgtttcc attatcaagg actacagagg aaagatcgaa
      361 actgaactca gcaaaatctg tgatggaata ctcaatcttc tggattctca ccttgttccc
      421 actgcatctt tggccgagtc caaagtcttt tacctcaaaa tgaaaggaga ttaccacagg
      481 taccttgctg agtttaagac tggagctgag aggaaagaag ctgctgagag cactctggtt
      541 gcttacaagt cagctcagga tattgcactt gctgatttag ctcctactca tccgattaga
      601 ctgggacttg ctcttaactt ctctgtcttc tactacgaga ttctcaactc acctgatcgt
      661 gcctgcagtc tcgcaaaaca ggcttttgat gaggccattt ctgagctgga tacattagga
      721 gaagaatcat acaaagacag tacgttgata atgcaacttc tccgtgacaa tctgaccctt
      781 tggaactctg acatcaatga tgaggcgggc ggtgatgaga tcaaggag
//
