LOCUS       sequence-63905-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Sehnke PC, Laughner B, Cardasis H, Powell D, Ferl RJ
  TITLE     Exposed loop domains of complexed 14-3-3 proteins contribute to 
            structural diversity and functional specificity.
  JOURNAL   Plant Physiol. 2006 Feb;140(2):647-60. Epub 2006 Jan 11.
  PUBMED    16407442
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006-02"; volume: "140(2)"; pages: "647-60. Epub 
            2006 Jan 11"
COMMENT     Sequence Label: pET15b-At 14-3-3 lambda
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..82
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag
       61 cggcctggtg ccgcgcggca gccatatggc ggcgacatta ggcagagacc agtatgtgta
      121 catggcgaag ctcgccgagc aggcggagcg ttacgaagag atggttcaat tcatggaaca
      181 gctcgttaca ggcgctactc cagcggaaga gctcaccgtt gaagagagga atctcctctc
      241 tgttgcttac aaaaacgtga tcggatctct acgcgccgcc tggaggatcg tgtcttcgat
      301 tgagcagaag gaagagagta ggaagaacga cgagcacgtg tcgcttgtca aggattacag
      361 atctaaagtt gagtctgagc tttcttctgt ttgctctgga atccttaagc tccttgactc
      421 gcatctgatc ccatctgctg gagcgagtga gtctaaggtc ttttacttga agatgaaagg
      481 tgattatcat cggtacatgg ctgagtttaa gtctggtgat gagaggaaaa ctgctgctga
      541 agataccatg ctcgcttaca aagcagctca ggatatcgca gctgcggata tggcacctac
      601 tcatccgata aggcttggtc tggccctgaa tttctcagtg ttctactatg agattctcaa
      661 ttcttcagac aaagcttgta acatggccaa acaggctttt gaggaggcca tagctgagct
      721 tgacactctg ggagaggaat cctacaaaga cagcactctc ataatgcagt tgctgaggga
      781 caatttaacc ctttggacct ccgatatgca ggagcagatg gacgaggcct gaggatctag
      841 atgaaggggg ggagggttgt tacgcgatgt ttctgccacc aaatcgatct caaaatcccc
      901 ataacctttg ctcaaaa
//
