LOCUS       sequence-63935-e        5704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pSABAD505
FEATURES             Location/Qualifiers
     source          1..5704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(39..917)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        944..1228
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1151..1170
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             1255..1277
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1318..1346)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2352..2372
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(2428..2445)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2597..2683
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2775..2802
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2820..2911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2912..2980,2981..3772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3130..3149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3814..4269
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3901..3920)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4111..4132
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      4380..4968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4869..4888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5122..5139
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5154..5294
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5220..5239)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5380..5402
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5541..5560
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca
       61 tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt
      121 ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg
      181 ataggcatcc gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc
      241 cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac
      301 aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg
      361 atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc
      421 gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag
      481 cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg
      541 tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat
      601 tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc
      661 ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg
      721 caaacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga
      781 atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca
      841 atcggcgtta aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca
      901 ttttgcgctt cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca
      961 tattgcatca gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg
     1021 gtaaccccgc ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg
     1081 cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc
     1141 acactttgct atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt
     1201 ttatcgcaac tctctactgt ttctccatac ccgttttttt gggctagaaa taattttgtt
     1261 taactttaag aaggagatat acatatggct agcaaaggag aagaactttt cactggagtt
     1321 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtccgcgga
     1381 gagggtgaag gtgatgctac aaacggaaaa cttaccctta aatttatttg cactactgga
     1441 aaactacctg ttccatggcc aacactagtt actactttat gtttaagcta tgaaacggaa
     1501 atattgacag tagaatatgg attattaccg attggtaaaa ttgtagaaaa gcgcatcgaa
     1561 tgtactgttt atagcgttga taataatgga aatatttata cacaacctgt agcacaatgg
     1621 cacgatcgcg gagaacaaga ggtgtttgag tattgtttgg aagatggttc attgattcgg
     1681 gcaacaaaag accataagtt tatgactgtt gatggtcaaa tgttgccaat tgatgaaata
     1741 tttgaacgtg aattggattt gatgcgggtt gataatttgc cgaatatcaa aatagccaca
     1801 cgtaaatatt taggcaaaca aaatgtctat gacattggag ttgagcgcga ccataatttt
     1861 gcactcaaaa atggcttcat agcttctaat tgttatggtg ttcaatgctt ttctagatat
     1921 ccggatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     1981 gaacgcacta tatctttcaa agatgacggg aactacaaga cgcgtgctga agtcaagttt
     2041 gaaggtgata cccttgttaa tcgtatcgag ttaaaaggta ttgattttaa agaagatgga
     2101 aacattctcg gacacaaact cgagtacaac tataactcac acaatgtata catcacggca
     2161 gacaaacaaa agaatggaat caaagctaac ttcaaaattc gccacaacat tgaagatgga
     2221 tccgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     2281 ttaccagaca accattacct gtcgacacaa tctgcccttt cgaaagatcc caacgaaaag
     2341 cgtgaccaca tggtccttct tgagtttgta actgctgctg ggttgactta agcttggctg
     2401 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
     2461 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
     2521 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtccccca tgcgagagta
     2581 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtgc aaagactggg cctttcgttt
     2641 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     2701 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     2761 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     2821 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2881 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2941 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3001 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3061 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3121 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     3181 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3241 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3301 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3361 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3421 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     3481 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3541 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3601 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3661 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3721 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3781 ccaagtttac tcatatatac tttagattga tttacgcgcc ctgtagcggc gcattaagcg
     3841 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3901 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3961 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4021 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4081 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4141 tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4201 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     4261 ttacaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4321 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4381 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4441 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4501 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4561 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4621 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4681 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4741 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     4801 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4861 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4921 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4981 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5041 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5101 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     5161 cggtattttc tccttacgca tctgtgcggt atttcacacc ggatatggtg cactctcagt
     5221 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     5281 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     5341 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     5401 ggttttcacc gtcatcaccg aaacgcgcga ggcagcaagg agatggcgcc caacagtccc
     5461 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     5521 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     5581 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctaattctc atgtttgaca
     5641 gcttatcatc gatgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct
     5701 atgc
//
