LOCUS       sequence-63941-e        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Piatkevich KD, Subach FV, Verkhusha VV
  TITLE     Far-red light photoactivatable near-infrared fluorescent proteins 
            engineered from a bacterial phytochrome.
  JOURNAL   Nat Commun. 2013 Jul 10;4:2153. doi: 10.1038/ncomms3153.
  PUBMED    23842578
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-07-10"; volume: "4:2153. doi"; pages: " 
            10.1038/ncomms3153"
COMMENT     Sequence Label: pBAD/HisB-PAiRFP1
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             84..101
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             105..137
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(141..149,150..164)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttg ggctaacagg
       61 aggaattaac catggggggt tctcatcatc atcatcatca tggtatggct agcatgactg
      121 gtggacagca aatgggtcgg gatctgtacg acgatgacga taaggatccg agctcgagat
      181 ctatggcatc cacggactat catgtcgacc tgaccaattg cgaccgcgaa cccattcata
      241 ttccaggata tattcaaccg catggctgcc tcatcgcctg cgataacgcc atgcgaatgg
      301 tgctgcggca ttccgaaaac tgcggcgaac tgctgggact tgagggcgat ctcaacggca
      361 ggaccgccga ggatgtgctc ggcaaaaagc tcgtccacga tctccgcaac gcgctcaccg
      421 tcaccggtag aaccacgcgc cccgccatgc tgcctgcaat ggaaacaagc gacggccgca
      481 gcttcgatat ttcactccat cgctacaaat ccaccaccat catcgaattc gagccctcgg
      541 gcagcgacgc gcagccgctc gacacggcgc gcaagatggt ggaccgcatc cgcgaagccg
      601 acagggtcga aagcctgatc tccagaacca cacgcctggt aaaggcgacg ctgggttacg
      661 accgggtgct gatctaccgc ttcctggaag acggcgccgg caaggtcgtg tcggaggcca
      721 agcagcccga gctggagagc tttctcgggc aatatttccc cgccagcgat attccacagc
      781 aggctcgcgt actttatctc aagaacacct tgcgtatcat ctccgatgcc agcagcaccc
      841 ccattccggt cctaccagcc gtcgatgtct ccggtgagcc gctcgatctc tcttacgcgc
      901 atttgcgcag cttctcgccc atccattgcg aatatctccg caatatgggt gttgccgcct
      961 ccatgtcgat ttctgtgatt gtcgatggcg ccctgtgggg cctgat
//
