LOCUS       sequence-63943-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Piatkevich KD, Subach FV, Verkhusha VV
  TITLE     Far-red light photoactivatable near-infrared fluorescent proteins 
            engineered from a bacterial phytochrome.
  JOURNAL   Nat Commun. 2013 Jul 10;4:2153. doi: 10.1038/ncomms3153.
  PUBMED    23842578
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-07-10"; volume: "4:2153. doi"; pages: " 
            10.1038/ncomms3153"
COMMENT     Sequence Label: pBAD/HisB-PAiRFP2
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..96
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             100..132
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(136..144,145..159)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa
       61 ttaaccatgg ggggttctca tcatcatcat catcatggta tggctagcat gactggtgga
      121 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccgagctc gagatctatg
      181 gcatccacgg actatcatgt cgacctgacc aattgcgacc gcgaacccat tcatattcca
      241 ggatatattc aaccgcatgg ctgcctcatc gcctgcgata acgccatgcg aatggtgctg
      301 cggcattccg aaaactgcgg cgaactgctg ggacttgagg gcgatctcaa cggcaggacc
      361 gccgaggatg tgctcggcaa aaggctcgtc catgatctcc gcaacgcgct caccgtcacc
      421 ggtaaaacca cgcgccccgc catgctgccc gcaatggaaa caagcgacgg ccgcagcttc
      481 gatatttcac tccatcgcta caaatccacc accatcatcg aattcgagcc ctcggacagc
      541 gacacgcagc cgctcggcac ggcgcgcaag atggtggacc gcatccgcga agccgacagc
      601 gtcgaaagcc tgatctccag aaccacacgc ctggtaaagg cgacgctggg ttacgaccgt
      661 gtgctgatct accgcttcga ggaagacggc gccggcaagg tcgtgtcgga ggccaagcag
      721 cccgagctgg agagctttct cgggcaatat ttccccgcca gcgatattcc acagcaggct
      781 cgcgcacttt atctcaagaa caccttgcgc atcatctccg atgccagcgg cacccccatt
      841 ccggtcctgc cagccgtcga tgtctccggt gagccgctcg atctctctta cgcgcatttg
      901 cgcaacttct cgcccatcca ttgcgaatat ctccgcaata tgggtgttgc cgcctccatg
      961 tcgatttctg tgattgtcga tgacgccctg tgggg
//
