LOCUS       sequence-63987-e        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Westermann B, Neupert W
  TITLE     Mitochondria-targeted green fluorescent proteins: convenient tools 
            for the study of organelle biogenesis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2000 Nov;16(15):1421-7.
  PUBMED    11054823
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2000-11"; volume: "16(15)"; pages: "1421-7"
COMMENT     Sequence Label: pYX113-mtGFP
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             243..959
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSAALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(273..301)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     899..919
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ccggaatcga attcatggcc tccactcgtg tcctcgcctc tcgcctggcc tcccggatgg
       61 ctgcttccgc caaggttgcc cgccctgctg tccgcgttgc tcaggtcagc aagcgcacca
      121 tccagactgg ctcccccctc cagaccctca agcgcaccca gatgacctcc atcgtcaacg
      181 ccaccacccg ccaggctttc cagaagcgcg cctactcttc cggatccccg ggtaccagat
      241 ctatgagtaa gggtgaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      301 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      361 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      421 ttgtcactac tttcacttat ggtgttcaat gcttttcaag atacccagat catatgaaac
      481 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      541 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      601 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      661 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      721 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      781 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      841 acctgtccac acaatctgct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
      901 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
      961 aaaagcttac gcgtgggacc ggggtatccg tatgatggtg cctgactacg catgatatct
     1021 cgagctcagc tagctaactg aataaggaac aatgaacgtt tttcccttcc ctggtcccag
//
