LOCUS       sequence-63995-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Westermann B, Neupert W
  TITLE     Mitochondria-targeted green fluorescent proteins: convenient tools 
            for the study of organelle biogenesis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2000 Nov;16(15):1421-7.
  PUBMED    11054823
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2000-11"; volume: "16(15)"; pages: "1421-7"
COMMENT     Sequence Label: pYES-mtGFP
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..41
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        22..40
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(312..340)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggatcggact actagcagct gtaatacgac tcactatagg gaatattaag cttatggcct
       61 ccactcgtgt cctcgcctct cgcctggcct cccggatggc tgcttccgcc aaggttgccc
      121 gccctgctgt ccgcgttgct caggtcagca agcgcaccat ccagactggc tcccccctcc
      181 agaccctcaa gcgcacccag atgacctcca tcgtcaacgc caccacccgc caggctttcc
      241 agaagcgcgc ctactcttcc ggatccccgg gtaccagatc tatgagtaag ggtgaagaac
      301 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      361 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
      421 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcacttatg
      481 gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg
      541 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
      601 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
      661 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
      721 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
      781 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
      841 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatct
//
