LOCUS       sequence-64005-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Westermann B, Neupert W
  TITLE     Mitochondria-targeted green fluorescent proteins: convenient tools 
            for the study of organelle biogenesis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2000 Nov;16(15):1421-7.
  PUBMED    11054823
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2000-11"; volume: "16(15)"; pages: "1421-7"
COMMENT     Sequence Label: pYES-mtBFP
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(292..320)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggactactag cagctgtaat acgactcact atagggaata ttaagcttat ggcctccact
       61 cgtgtcctcg cctctcgcct ggcctcccgg atggctgctt ccgccaaggt tgcccgccct
      121 gctgtccgcg ttgctcaggt cagcaagcgc accatccaga ctggctcccc cctccagacc
      181 ctcaagcgca cccagatgac ctccatcgtc aacgccacca cccgccaggc tttccagaag
      241 cgcgcctact cttccggatc tatgagtaag ggtgaagaac ttttcactgg agttgtccca
      301 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      361 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      421 cctgttccat ggccaacact tgtcactact ttctctcatg gtgttcaatg cttttcaaga
      481 tacccagatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      541 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      601 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      661 ggaaacattc ttggacacaa attggaatac aactttaact cacacaatgt atacatcatg
      721 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      781 ggaagcgttc aactagcaga ccattatcaa caaaatactc
//
