LOCUS       sequence-64034-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN149
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(82..123)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(366..390)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(413..616)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(442..462)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        617..920
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt gtcgacttaa cgcgccaccg
       61 gttagcgcta gctcattact acgtagaatc gagaccgagg agagggttag ggataggctt
      121 accaaccact ttgtacaaga aagttgggta tgatttttca tgtgaagatt taggtgtttc
      181 tccatcattt tcctcttcct catcctcttc acaatcccca tctgtttgct gttccagttc
      241 ctcttttgtt atcatttcaa aatcatttcc atccagaaaa acctgatcat catgtggctc
      301 aagtttgaat tttttcttag tttccttctt tttaggagtt cctggagttt cctccttttt
      361 ggacatgcca acttttttgt acaaacttgt tgatcccgac agttagccag agagctctgc
      421 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
      481 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
      541 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
      601 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
      661 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
      721 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
      781 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
      841 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
      901 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
      961 ttgattac
//
