LOCUS       sequence-64038-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, 
            Voytas DF
  TITLE     TALENs enable efficient plant genome engineering.
  JOURNAL   Plant Physiol. 2012 Nov 2.
  PUBMED    23124327
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2012 Nov 2."
COMMENT     Sequence Label: pZHY402
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..262)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(302..553)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVMLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMEELYK"
     primer_bind     complement(344..365)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     693..712
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgggcccacg atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc
       61 gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa
      121 cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca
      181 acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt
      241 attgccaaat gtttgaacga tcggggaaat tcgagctcta aaggtctccc gggaaccgcg
      301 gcttgtacag ctcttccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
      361 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactggtag ctcaggtagt
      421 ggttgtcggg cagcatcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
      481 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
      541 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
      601 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      661 gtgtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
      721 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
      781 tcatgtggtc ggggta
//
