LOCUS       sequence-64069-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength GCaMP2-based genetically encoded calcium 
            indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Tian L, Hires SA, Mao T, Huber D, Chiappe ME, Chalasani SH, Petreanu
            L, Akerboom J, McKinney SA, Schreiter ER, Bargmann CI, Jayaraman V, 
            Svoboda K, Looger LL.
  TITLE     Imaging neural activity in worms, flies and mice with improved GCaMP
            calcium indicators.
  JOURNAL   Nat Methods. 2009 Dec;6(12):875-81.
  PUBMED    19898485
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2009-12"; volume: "6(12)"; pages: "875-81"
COMMENT     Sequence Label: G-CaMP3
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..92
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             96..128
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(132..140,141..155)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             183..239
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     452..473
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(579..600)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(840..859)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgcca
       61 ccatgatggg ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc
      121 aaatgggtcg ggatctgtac gacgatgacg ataaggatct cgccaccatg gtcgactcat
      181 cacgtcgtaa gtggaataag acaggtcacg cagtcagagc tataggtcgg ctgagctcac
      241 tcgagaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttcaaga
      301 tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc
      361 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccaaac
      421 tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      481 ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga
      541 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      601 taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc tacggcaagc
      661 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      721 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      781 acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc ttcttcaagg
      841 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      901 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgg
//
